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atomic force microscopy (AFM)
Click for insertion detail, resultant modification
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Discoveries: RAPID EVOLUTION OF A PROTEIN
IN-VITRO BY DNA SHUFFLING
Our research is focused on the molecular mechanisms of genetic recombination,
with the long-term objective being the reconstitution of in-vitro systems
that accurately reproduce the cellular processes. We are characterizing
the biochemical properties of proteins essential to homologous recombination,
in prokaryotes, eukaryotes, and Archaea.
These ideas are all accepted by the overwhelming bulk of scientists,
with the possible exception of gradualism, which in its purest manifestation
has been challenged by Gould’s and Eldrege’s concept of punctuated
equilibrium. The other ideas now form a set that can justifiably be
called the fact of evolution.
Transposons and Retrotransposons
Transpositional recombination is the process that a mobile element is
inserted into a target DNA. It may occur by one of two mechanisms:
(1) directly as DNA, (2) through RNA. The mobile elements that
transpose through DNA are called transposons and those via RNA are referred
to as retrotransposons.
DNA shuffling is a method for in vitro homologous recombination of
pools of selected mutant genes by random fragmentation and polymerase
chain reaction (PCR) reassembly.
Genetic recombination is the transfer of DNA from one organism to another.
The transferred donor DNA may then be integrated into the recipient's
nucleoid by various mechanisms. Through Transposon Technology and DNA
shuffling a recombination in cell property occurs.
Flp recombinase promotes site-specific DNA recombination in embryonic
stem cells and transgenic swine. Broad Flp expression is compatible
with normal development, suggesting that Flp can be used to catalyze
recombination in most cell types. These properties indicate that Flp
can be exploited to make prescribed alterations in both swine and primate
genome -- to include the Homo Sapian Sapian..
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crossover experiment with undesirable results
Frolic
in Paradise
Once upon a time there was a big jug of cider sitting in a cool place.
In this jug were little yeasties. The yeastie needed to eat. Being
hungry for food and procreation, the little yeasties slurped cider
sugars and they shat alcohol and they procreated and procreated, as
it is, exponentially. These little critters operated as if there were
limitless sugars and space to spread their way of life; it was just
like paradise. So, by and by, they ate and shat and shat and ate and
spread throughout the tasty cider soup until the sugar was gone and
they began to drown in their own shit; the alcohol excrement was,
of course, poison to the little critters. All the little yeasties
died and their little world was turned to shit.
Had the little yeastie been able to forecast the resultant alchemy
of this behavior they might have taken a different tack. Had the little
yeasties engaged in experimentation and research, intervening variables
might have been implemented to change their paradigm.
If the little yeasties were equipped with genetic engineering, they
might have programmed their diet to eat alcohol and shit sugar, reversing
the process that was their demise. Had the organism gotten a handle
on its ability to procreate the cider environment could have supported
the frolic and feasting of the yeasty for some length of time. This
time would have been valuable to the survival of the little critter;
there would have been time for research into exploration beyond the
cider jug, exploit other cider jugs and spread the Good News to other
parts of the realm. It's through this fairy tale that we at Scari
Research Institute have postulated a template for the human condition.
(If this were a anthropocentric story and we being the good guys,
this would be where we'd come in to save the day, but it's a story
about yeasties)
Conclusion: Had the yeastie been inbued with cognition and
science beyond natural selection they could have taken counter measures
that would secure their fate, perserve the realm and continue their
frolic.
parson Carson Gallant Charoot
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Upon specific stimulation, cells in the G0 phase may re-enter
the G1 phase.
Note:
Codes are under license and are owned with intellectual rights held by
licensee
It's not been a bed of roses, we have had setbacks,
and miss-steps; not all of our research has born usable fruit. An omnipresent
dissapointment has been Large Offspring Syndrome; a condition that is
now mitigated by massive doses of steroids and hormone therapy after delivery.
For us at SCARI "life starts at conception and ends at birth,"
we have no interest in postnatal depression responsibilities.
Tagging Errors
1272 bases (including the stop codon) Tagging errors were responsible
for our mistaken assurance of success. The result of this miscalculation
has caused a reassessment of our original data.
ccaat ttggtatatt atgtaaaaca ccacctaagg tgcttgttcg tcagatgccatttgtg
gaaaggtttg aaagaccttc aggtgagaaa atagcattat gtgctgctga actaacctat ttatgttgga
tgattacaca taacggaaca gcaatcaaga gagccacatt catgagctat aatactatca taagcaattc
gctgagtttc gatattgtca ataaatcact ccagtttaaa tacaagacgc aataagcaac
aattctggaa gcctcattaa agaaattgat tcctgcttgg gaatttacaa ttattcctta ctatggacaa
aaacatcaat ctgatatcac tgatattgta agtagtttgc aattacagtt cgaatcatcg gaagaagcag
ataagggaaa tagccacagt aaaaaaatgc ttaaagcact tctaagtgag
ggtgaaagca tctgggagat cactgagaaa atactaaatt cgtttgagta tacttcgaga
tttacaaaaa caaaaacttt ataccaattc ctcttcctag ctactttcat caattgtgga agattcagcg
atattaagaa cgttgatccg aaatcattta aattagtcca aaataagtat ctgggagtaa taatccagtg
tttagtgaca gagacaaaga caaccgatag taggcacata
tacttcttta gcgcaagggg taggatcgat ccacttgtat atttggatga atttttgagg aattctgaac
cagtcctaaa acgagtaaat aggaccggca attcttcaag caataaacag gaataccaat tattaaaaga
taacttagtc agatcgtaca ataaagcttt gaagaaaaat gcgccttatt caatctttgc tataaaaaat
gtcccaaaat ctcacattgg aagacatttg atgacctcat ttctttcaat gaagggccta
acggagttga ctaatgttgt gggaaattgg agcgataagc gtgcttctgc cgtggccagg acaacgtata
ctcatcagat aacagcaata cctgatcact acttcgcact agtttctcgg tactatgcat atggcccaat
atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta
aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag gtactagact
acctttcatc ctacataaat agacgcatat aa
Subsequent typing has not been so error prone.
DNA at work: With Scientific Relish we at SCARI
Genome have sliced through the helix eliminating the skree and clutter
of normal plodding research, adding a level of excitement and intensity
of our research, research that would, under normal labrotory norms, be
droll and boring.
Tagger reorganization: Taggers employed for sequence annotation have
been reorganized into a phalanx of devoted genome sequencing technicians.
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